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Proceedings Paper

Femtosecond laser-induced calcium release in neural-type cells
Author(s): Nicholas Isaac Smith; Shigeki Iwanaga; Taro Beppu; Katsumasa Fujita; Osamu Nakamura; Satoshi Kawata
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Paper Abstract

Here we show that femtosecond laser irradiation can be used to evoke dynamic calcium concentration changes in living cells. The relatively localized interaction that results from the two-photon absorption process allows the release of calcium from intracellular stores in cells in vitro. The self-catalytic response to calcium elevation in a cell can increase the initial release of calcium further so that the entire cell undergoes a rise in cytosolic calcium concentration (i.e. a calcium wave). The calcium stimulation was observed in HeLa (non-excitable) and PC12 (excitable) cells, and could be seen to occur inside a range of power levels between approximately 20 to 80mW. The observation of direct calcium release by femtosecond laser which leads to a calcium wave in the cell has implications for photolytic calcium uncaging experiments since it could be a competing, or even dominant factor in some experiments using caged calcium for the generation of calcium waves.

Paper Details

Date Published: 28 April 2005
PDF: 6 pages
Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); doi: 10.1117/12.596923
Show Author Affiliations
Nicholas Isaac Smith, Osaka Univ. (Japan)
Shigeki Iwanaga, Osaka Univ. (Japan)
Taro Beppu, Osaka Univ. (Japan)
Katsumasa Fujita, Osaka Univ. (Japan)
Osamu Nakamura, Osaka Univ. (Japan)
Satoshi Kawata, Osaka Univ. (Japan)
RIKEN-The Institute of Physical and Chemical Research (Japan)

Published in SPIE Proceedings Vol. 5705:
Nanobiophotonics and Biomedical Applications II
Alexander N. Cartwright; Marek Osinski, Editor(s)

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