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Proceedings Paper

A novel node-structural map for angiogenesis analysis
Author(s): Yizhi Xiong; Tong Zhao; Dodanim Talavera; Rocio S. Honigmann; Daniel L. Farkas
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Paper Abstract

We introduce a novel node-structural map method for digital image-based analysis of angiogenesis, and apply it to quantitate the effect of pro-angiogenic (e.g. VEGF) and anti-angiogenic (e.g. anti-VEGF) treatments on blood vessel patterns in the quail chorioallantoic membrane (CAM) in vivo model. After vessel segmentation and skeletonization, a node-structural map is derived to represent the structural property of each pixel in the skeletonized image, such as end nodes (root and leaf), branch and furcation/junction nodes. By combining the node-structural map with vascular thickness map, our proposed method provides more detailed morphometric and structural measurement of vascular angiogenesis, such as average diameter, average branch length, branch thickness distribution, density of branch and furcation nodes, etc. Furthermore, the concept of vascular generations will be proposed. Accordingly, more detailed measurements related to the generations of the vascular tree can be easily evaluated. From the quantitative analysis results, it correctly shows the fact that VEGF/anti-VEGF can modify the blood vessel pattern and increase/decrease the vessel density in CAM significantly, compared to the phosphate-buffered saline treated controls.

Paper Details

Date Published: 29 April 2005
PDF: 9 pages
Proc. SPIE 5747, Medical Imaging 2005: Image Processing, (29 April 2005); doi: 10.1117/12.596443
Show Author Affiliations
Yizhi Xiong, Cedars-Sinai Medical Ctr. (United States)
Tong Zhao, Carnegie Mellon Univ. (United States)
Dodanim Talavera, Cedars-Sinai Medical Ctr. (United States)
Rocio S. Honigmann, Cedars-Sinai Medical Ctr. (United States)
Daniel L. Farkas, Cedars-Sinai Medical Ctr. (United States)


Published in SPIE Proceedings Vol. 5747:
Medical Imaging 2005: Image Processing
J. Michael Fitzpatrick; Joseph M. Reinhardt, Editor(s)

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