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Proceedings Paper

Novel approach to process protein crystals using deep-UV laser
Author(s): Hiroshi Kitano; Atsushi Sato; Atsutoshi Murakami; Hiroaki Adachi; Hiroyoshi Matsumura; Kazufumi Takano; Tsuyoshi Inoue; Yusuke Mori; Masaaki Doi; Takatomo Sasaki
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Paper Abstract

We propose new application of a pulsed deep-UV laser to processing biological macromolecule crystals. Single crystals of model protein, hen egg white lysozyme, were processed by deep-UV laser irradiation at 193 nm and 266 nm. Desired laser ablation was achieved using the 193-nm light source. A crystallinity of the processed crystals was evaluated by X-ray diffraction pattern recording. The crystal diffracted beyond a 1.9 Å resolution, which was the same as that of the as-grown crystal obtained under identical growth conditions. In addition, we observed that a diffraction pattern from a cracked protein crystal was considerably improved by eliminating the damaged section with the 193-nm laser ablation. A processing tool for protein crystals currently employed, such as a micro needle or knife, suffers from low reproducibility and poor accuracy. The technique using deep-UV laser pulses will be a powerful tool for processing very fragile protein crystals.

Paper Details

Date Published: 8 October 2004
PDF: 5 pages
Proc. SPIE 5662, Fifth International Symposium on Laser Precision Microfabrication, (8 October 2004); doi: 10.1117/12.596331
Show Author Affiliations
Hiroshi Kitano, Osaka Univ. (Japan)
Nikon Corp. (Japan)
Atsushi Sato, Osaka Univ. (Japan)
Atsutoshi Murakami, Osaka Univ. (Japan)
Hiroaki Adachi, Osaka Univ. (Japan)
Hiroyoshi Matsumura, Osaka Univ. (Japan)
Kazufumi Takano, Osaka Univ. (Japan)
Japan Science and Technology Agency (Japan)
NEDO (Japan)
Tsuyoshi Inoue, Osaka Univ. (Japan)
Japan Science and Technology Agency (Japan)
Yusuke Mori, Osaka Univ. (Japan)
Masaaki Doi, Nikon Corp. (Japan)
Takatomo Sasaki, Osaka Univ. (Japan)

Published in SPIE Proceedings Vol. 5662:
Fifth International Symposium on Laser Precision Microfabrication
Isamu Miyamoto; Henry Helvajian; Kazuyoshi Itoh; Kojiro F. Kobayashi; Andreas Ostendorf; Koji Sugioka, Editor(s)

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