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Proceedings Paper

High-sensitivity confocal imaging with bilateral scanning and CCD detectors
Author(s): G. J. Brakenhoff; Koen Visscher
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Paper Abstract

The high quantum efficiency and high red sensitivity of a charge coupled device (CCD) make it a very suitable detector for confocal fluorescence imaging, especially for applications in biology, where short wavelength illumination may cause undesirable radiation damage. In addition, its high dynamic range matches the high contrast imaging inherent in confocal microscopy, and enables one to record small intensity differences if this dynamic range is well exploited. Applications shown include imaging with bleaching rate and non-linear fluorescence excitation as parameters. The bilateral scan technique permits effective confocal imaging using this type of device. CCDs can be used for confocal imaging both in high speed (up to real- time) applications as well as in the integration mode, where the high sensitivity application areas for this confocal technique are present.

Paper Details

Date Published: 26 June 1992
PDF: 11 pages
Proc. SPIE 1660, Biomedical Image Processing and Three-Dimensional Microscopy, (26 June 1992); doi: 10.1117/12.59582
Show Author Affiliations
G. J. Brakenhoff, Univ. of Amsterdam (Netherlands)
Koen Visscher, Univ. of Amsterdam (Netherlands)

Published in SPIE Proceedings Vol. 1660:
Biomedical Image Processing and Three-Dimensional Microscopy
Raj S. Acharya; Carol J. Cogswell; Dmitry B. Goldgof, Editor(s)

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