Share Email Print

Proceedings Paper

High-resolution confocal transmission microscope: Part II - determining image position and correcting aberrations
Author(s): John W. O'Byrne; Carol J. Cogswell
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

In order to produce high resolution images of complex biological specimens in a confocal transmission microscope, two phenomena must be overcome. Firstly, non-uniform refractive index variations in the specimen cause deflection of the focused image spot in three- dimensional space which presents a problem for the usual confocal approach of imaging onto a fixed pinhole. The second effect is optical aberrations (especially spherical aberration) which arise as a natural function of imaging through the thickness of a refractive specimen. This paper discusses how both effects may be monitored and overcome, while still providing confocal imaging, using a CCD array as the detector

Paper Details

Date Published: 26 June 1992
PDF: 9 pages
Proc. SPIE 1660, Biomedical Image Processing and Three-Dimensional Microscopy, (26 June 1992); doi: 10.1117/12.59580
Show Author Affiliations
John W. O'Byrne, Univ. of Sydney (Australia)
Carol J. Cogswell, Univ. of Sydney (Australia)

Published in SPIE Proceedings Vol. 1660:
Biomedical Image Processing and Three-Dimensional Microscopy
Raj S. Acharya; Carol J. Cogswell; Dmitry B. Goldgof, Editor(s)

© SPIE. Terms of Use
Back to Top