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Proceedings Paper

Depth-resolved fluorescence of human ectocervical tissue
Author(s): Yicong Wu; Peng Xi; Tak-Hong Cheung; So Fan Yim; Mei-Yung Yu; Jianan Y. Qu
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Paper Abstract

The depth-resolved autofluorescence of normal and dysplastic human ectocervical tissue within 120um depth were investigated utilizing a portable confocal fluorescence spectroscopy with the excitations at 355nm and 457nm. From the topmost keratinizing layer of all ectocervical tissue samples, strong keratin fluorescence with the spectral characteristics similar to collagen was observed, which created serious interference in seeking the correlation between tissue fluorescence and tissue pathology. While from the underlying non-keratinizing epithelial layer, the measured NADH fluorescence induced by 355nm excitation and FAD fluorescence induced by 457nm excitation were strongly correlated to the tissue pathology. The ratios between NADH over FAD fluorescence increased statistically in the CIN epithelial relative to the normal and HPV epithelia, which indicated increased metabolic activity in precancerous tissue. This study demonstrates that the depth-resolved fluorescence spectroscopy can reveal fine structural information on epithelial tissue and potentially provide more accurate diagnostic information for determining tissue pathology.

Paper Details

Date Published: 1 April 2005
PDF: 6 pages
Proc. SPIE 5692, Advanced Biomedical and Clinical Diagnostic Systems III, (1 April 2005); doi: 10.1117/12.593826
Show Author Affiliations
Yicong Wu, Hong Kong Univ. of Science and Technology (Hong Kong China)
Peng Xi, Hong Kong Univ. of Science and Technology (Hong Kong China)
Tak-Hong Cheung, Chinese Univ. of Hong Kong Prince of Wales Hospital (Hong Kong China)
So Fan Yim, Chinese Univ. of Hong Kong Prince of Wales Hospital (Hong Kong China)
Mei-Yung Yu, Chinese Univ. of Hong Kong Prince of Wales Hospital (Hong Kong China)
Jianan Y. Qu, Hong Kong Univ. of Science and Technology (Hong Kong China)


Published in SPIE Proceedings Vol. 5692:
Advanced Biomedical and Clinical Diagnostic Systems III
Tuan Vo-Dinh; Warren S. Grundfest; David A. Benaron; Gerald E. Cohn, Editor(s)

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