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Proceedings Paper

Comparison of in-vitro photosensitizer efficiencies
Author(s): Barbara E. Krammer
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Paper Abstract

Due to lack of optimal properties of the officially admitted photosensitizer hematoporphyrin derivative (HPD), new sensitizers with better properties, such as the main absorption in the visible red range, higher chemical stability, higher tumor selectivity, and lower skin and dark toxicity, have to be found and investigated. In the present study, the effect of Photosan as active fraction of HPD was compared to Photofrin, HPD, HPD enriched with monomers (HPD-M) and polymers (MPD-P), to aluminum chloride tetrasulfonated phthalocyanine (AlClTSPc) and meso-tetraphenylporphyrin tetrasulfonate (TPPS4). Test parameters in fast screening tests were the membrane integrity of human skin fibroblasts, singlet oxygen and hydroxy radical formation, and absorption and fluorescence properties. The screening tests used were a modified trypan blue exclusion test, an oxygen consumption measuring set-up with polarographic electrodes (Clark-style) and absorption and fluorescence spectrophotometry. While HPD, HPD-M and Photofrin II were highly efficient in the membrane integrity testing compared to Photosan III, HPD-P and AlClTSPc showed less efficiency and TPPS almost no membrane related activity. When HPD and related compounds were taken, the efficiency of induction of the membrane damage was in general correlated with the formation of reactive oxygen species and with the monomer content of the substance.

Paper Details

Date Published: 6 May 1992
PDF: 7 pages
Proc. SPIE 1641, Physiological Monitoring and Early Detection Diagnostic Methods, (6 May 1992); doi: 10.1117/12.59377
Show Author Affiliations
Barbara E. Krammer, Univ. of Salzburg (Austria)

Published in SPIE Proceedings Vol. 1641:
Physiological Monitoring and Early Detection Diagnostic Methods
Thomas S. Mang, Editor(s)

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