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Proceedings Paper

Multicolor fluoroimmunoassays based on surface plasmon-coupled emission
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Paper Abstract

We present a new method for multi-color fluoroimmunoassays based on directional surface plasmon-coupled emission (SPCE). SPCE is coupling of excited fluorophores with a nearby thin metal film (silver) resulting in strongly directional emission into the underlying glass substrate. The angle at which the radiation propagates through the prism depends on emission wavelength and makes possible measurement of multiple analytes using multiple emission wavelengths. We demonstrated this possibility using two antibodies labeled with different fluorophores, binding to an antigen protein immobilized on the silver surface. We observed independent emission at a different angle on the glass prism, resulting of the surface binding of each antibody. This methodology can be readily extended to 3 or more fluorophores. This technology presents opportunity to develop highly sensitive multiplex assay format for biological agents' detection.

Paper Details

Date Published: 1 April 2005
PDF: 10 pages
Proc. SPIE 5692, Advanced Biomedical and Clinical Diagnostic Systems III, (1 April 2005); doi: 10.1117/12.593430
Show Author Affiliations
Evgenia G. Matveeva, Univ. of Maryland/Baltimore School of Medicine (United States)
Joanna Malicka, Univ. of Maryland/Baltimore School of Medicine (United States)
Ignacy Gryczynski, Univ. of Maryland/Baltimore School of Medicine (United States)
Joseph R. Lakowicz, Univ. of Maryland/Baltimore School of Medicine (United States)
Zygmunt Karol Gryczynski, Univ. of Maryland/Baltimore School of Medicine (United States)


Published in SPIE Proceedings Vol. 5692:
Advanced Biomedical and Clinical Diagnostic Systems III
Tuan Vo-Dinh; Warren S. Grundfest; David A. Benaron; Gerald E. Cohn, Editor(s)

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