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Proceedings Paper

Enhanced fluorescence of labeled proteins coated on silver nanoparticles
Author(s): Joanna Lukomska; Evgenia G. Matveeva; Ignacy Gryczynski; Robert W. Flower; Joanna Malicka; Joseph R. Lakowicz; Slawomir Makowiec; Zygmunt K. Gryczynski
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Paper Abstract

We have recently shown that metallic particles or colloids when deposited on the transparent surface can enhance fluorescence properties of nearby fluorophores. We obtained the fluorophore-metal colloid complexes that display significant fluorescence signal enhancement in solution. Silver nanoparticles (about 20-50 nm size) were synthesized as a stable yellow colloidal solution, and coated with proteins labeled with fluorophores. A several-fold amplification of the fluorescence signal in presence of colloid Ag nanoparticles in solution was observed. Such fluorophore-metal complex presents a unique opportunity for developing of new class of contrast agents for optical imaging and fluorescence based sensing. Solution of silver nanoparticles with enhanced fluorescence can be used in various assays such as DNA hybridization or immunoassays for high sensitivity detection.

Paper Details

Date Published: 31 March 2005
PDF: 7 pages
Proc. SPIE 5703, Plasmonics in Biology and Medicine II, (31 March 2005); doi: 10.1117/12.593426
Show Author Affiliations
Joanna Lukomska, Univ. of Maryland/Baltimore (United States)
Evgenia G. Matveeva, Univ. of Maryland/Baltimore (United States)
Ignacy Gryczynski, Univ. of Maryland/Baltimore (United States)
Robert W. Flower, Univ. of Maryland/Baltimore (United States)
New York Univ. (United States)
Joanna Malicka, Univ. of Maryland/Baltimore (United States)
Joseph R. Lakowicz, Univ. of Maryland/Baltimore (United States)
Slawomir Makowiec, Univ. of Maryland/Baltimore (United States)
Zygmunt K. Gryczynski, Univ. of Maryland/Baltimore (United States)


Published in SPIE Proceedings Vol. 5703:
Plasmonics in Biology and Medicine II
Tuan Vo-Dinh; Joseph R. Lakowicz; Zygmunt K. Gryczynski, Editor(s)

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