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Proceedings Paper

Optical monitor for microbial metabolism for hazardous waste application
Author(s): M. Ryan-Baker; Tuan Vo-Dinh; Guy D. Griffin; Gordon H. Miller; Jean Pierre Alarie; Robert S. Burlage; A. V. Palumbo; Dennis C. White; S. Herbes
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Paper Abstract

A remote detection system for in-situ monitoring of the bioluminescence produced by a microbial "reporter" strain grown in rotary film bioreactors has been developed. Two systems were developed: one using a low-temperature photomultiplier (PMT), and one using an avalanche photodiode. The photodiode system was preferred for its sensitivity and portability. Testing was performed on a recombinant constitutive light producing strain. The results indicate that the optical monitor is sensitive enough for direct microbial detection for hazardous waste application.

Paper Details

Date Published: 1 May 1992
PDF: 12 pages
Proc. SPIE 1637, Environmental and Process Monitoring Technologies, (1 May 1992); doi: 10.1117/12.59339
Show Author Affiliations
M. Ryan-Baker, Oak Ridge National Lab. (United States)
Tuan Vo-Dinh, Oak Ridge National Lab. (United States)
Guy D. Griffin, Oak Ridge National Lab. (United States)
Gordon H. Miller, Oak Ridge National Lab. (United States)
Jean Pierre Alarie, Oak Ridge National Lab. (United States)
Robert S. Burlage, Oak Ridge National Lab. (United States)
A. V. Palumbo, Oak Ridge National Lab. (United States)
Dennis C. White, Oak Ridge National Lab. (United States)
S. Herbes, Oak Ridge National Lab. (United States)


Published in SPIE Proceedings Vol. 1637:
Environmental and Process Monitoring Technologies
Tuan Vo-Dinh, Editor(s)

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