Share Email Print
cover

Proceedings Paper

Nonlinear interferometric vibrational imaging: optical ranging and spatial localization of CARS
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Diagnostic imaging trends are progressing toward the molecular level with the advent of molecular imaging techniques. Optical molecular imaging techniques that utilize targeted exogenous contrast agents or detect endogenous molecular signatures will significantly extend our ability to detect early pathological changes in biological tissue, and treat diseases early when they are most amenable to be cured. We have developed a technique called Nonlinear Interferometric Vibrational Imaging (NIVI) that takes advantage of the coherent nature of coherent anti-Stokes Raman scattering (CARS) processes and the coherent optical ranging and imaging capabilities of optical coherence tomography (OCT). OCT uses interferometry and heterodyne detection in the time or spectral domain to localize reflections of near-infrared light deep from within highly-scattering tissues. OCT has found wide biological and medical applications, and recently, molecular imaging methods have been developed. By utilizing the molecular-sensitivity of CARS, NIVI performs optical ranging and multi-dimensional molecular imaging with OCT-like optical systems, enabling the retrieval of not only χ(3) [chi(3)] amplitude but also phase information, the rejection of problematic non-resonant background four-wave-mixing signals, enhanced sensitivity from heterodyne detection, and a relaxation of the high-numerical aperture focusing requirements present in CARS microscopy. We present recent progress and advances in NIVI, including depth-ranging capabilities that extend significantly deeper than current CARS microscopy methods and are potentially more suitable for cross-sectional deep-tissue in vivo imaging.

Paper Details

Date Published: 30 March 2005
PDF: 10 pages
Proc. SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V, (30 March 2005); doi: 10.1117/12.592862
Show Author Affiliations
Stephen A. Boppart, Beckman Institute for Advanced Science and Technology (United States)
Univ. of Illinois/Urbana-Champaign (United States)
Daniel L. Marks, Beckman Institute for Advanced Science and Technology (United States)
Univ. of Illinois/Urbana-Champaign (United States)
Jeremy S. Bredfeldt, Beckman Institute for Advanced Science and Technology (United States)
Univ. of Illinois/Urbana-Champaign (United States)
Claudio Vinegoni, Beckman Institute for Advanced Science and Technology (United States)
Univ. of Illinois/Urbana-Champaign (United States)


Published in SPIE Proceedings Vol. 5700:
Multiphoton Microscopy in the Biomedical Sciences V
Ammasi Periasamy; Peter T. C. So, Editor(s)

© SPIE. Terms of Use
Back to Top