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Proceedings Paper

Monitoring cell profile in tissue engineered constructs by OCT
Author(s): Ying Yang; Pierre O. Bagnaninchi; Mairead A. Wood; Alicia J. El Haj; Elvire Guyot; Arnaud Dubois; Ruikang K. Wang
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Paper Abstract

Despite significant progress in tissue engineering over the last decade, the development of real-time, non-destructive tools for monitoring the development of engineered tissues remains a great challenge. To date, the evaluation of cell proliferation and extracellular matrix production in response to various culture conditions depends upon traditional DNA, RNA and protein analysis which requires extraction of cell components from constructs resulting in loss of tissue morphology and integrity. In this study, we report how optical coherence tomography (OCT) can be exploited to monitor cell profiles in real-time and in a non-destructive manner. Scaffolds made from poly(lactic acid) (PLLA) with various porosities were scanned by OCT. A local porosity analysis method has been developed to quantify the porosity change. The hypothesis is whether the local porosity analysis can correlate with the tissue growth within the scaffold following seeding of the cells within it. Bone cells have been grown in the PLLA scaffolds under different culture conditions. The OCT images of these scaffolds have been collected. It has been found that the porosity of the cultured scaffold-cell constructs reduced under different culture conditions compared to blank scaffolds. A decrease in light penetration depth in OCT images has also been observed. There existed a good relationship between the local porosity and tissue growth. It has been demonstrated that the mean local porosity based on OCT images can become a unique method to correlate and monitor tissue growth.

Paper Details

Date Published: 15 April 2005
PDF: 7 pages
Proc. SPIE 5695, Optical Interactions with Tissue and Cells XVI, (15 April 2005); doi: 10.1117/12.592752
Show Author Affiliations
Ying Yang, Keele Univ. (United Kingdom)
Pierre O. Bagnaninchi, Keele Univ. (United Kingdom)
Mairead A. Wood, Keele Univ. (United Kingdom)
Alicia J. El Haj, Keele Univ. (United Kingdom)
Elvire Guyot, Ecole Superieure de Physique et de Chimie Industrielles (France)
Arnaud Dubois, Ecole Superieure de Physique et de Chimie Industrielles (France)
Ruikang K. Wang, Cranfield Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 5695:
Optical Interactions with Tissue and Cells XVI
Steven L. Jacques; William P. Roach, Editor(s)

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