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Proceedings Paper

A novel PDMS microfluidic spotter for fabrication of protein chips and microarrays
Author(s): David A. Chang-Yen; David Myszka; Bruce K. Gale
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Paper Abstract

A novel PDMS microfluidic spotter system has been developed for the patterning of surface microarrays that require individually addressing each spot area and high probe density. Microfluidic channels are used to address each spot region and large spot arrays can be addressed in parallel. Fluorescence intensity measurement of dye-spotted samples compared to control and pipetted drops demonstrated a minimum of a three-fold increase in dye surface density. Surface plasmon resonance measurement of protein-spotted samples as compared to pin-spotted samples demonstrated an 86-fold increase in protein surface density. This novel spotter system can be applied to the production of high-throughput arrays in the fields of genomics, proteomics, immunoassays and fluorescence or luminescence assays.

Paper Details

Date Published: 22 January 2005
PDF: 11 pages
Proc. SPIE 5718, Microfluidics, BioMEMS, and Medical Microsystems III, (22 January 2005); doi: 10.1117/12.592151
Show Author Affiliations
David A. Chang-Yen, Univ. of Utah (United States)
David Myszka, Univ. of Utah (United States)
Bruce K. Gale, Univ. of Utah (United States)

Published in SPIE Proceedings Vol. 5718:
Microfluidics, BioMEMS, and Medical Microsystems III
Ian Papautsky; Isabelle Chartier, Editor(s)

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