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Proceedings Paper

High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera
Author(s): Sumin Han; Kang-Bin Im; Hwajoon Park; Dongsun Kim; Beop-Min Kim
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Paper Abstract

Using an acousto-optic beam deflector and a line scan camera, we constructed a non-mechanical, slit-scanning confocal microscope. It generates two-dimensional 512x512 images with speed of 60 frames/sec, which can be easily expanded, to 100 frames/sec. The measured axial and lateral resolutions of the system are 3.3 mm and <1 mm, respectively, with a 50X objective. This simple design can produce frames rates faster than other commercial products with comparable resolution, which may be useful for analysis of rapid interactions in various biological applications.

Paper Details

Date Published: 24 March 2005
PDF: 8 pages
Proc. SPIE 5701, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XII, (24 March 2005); doi: 10.1117/12.591465
Show Author Affiliations
Sumin Han, Yonsei Univ. (South Korea)
Kang-Bin Im, Yonsei Univ. (South Korea)
Hwajoon Park, Yonsei Univ. (South Korea)
Dongsun Kim, Coreware (South Korea)
Beop-Min Kim, Yonsei Univ. (South Korea)


Published in SPIE Proceedings Vol. 5701:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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