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Proceedings Paper

Cell damage during femtosecond optical trapping
Author(s): Kang-Bin Im; Sumin Han; Hwajoon Park; Dan Jin; Soo-Kie Kim; Beop-Min Kim
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Paper Abstract

Microparticles including biological cells were trapped and manipulated using a continuous wave tweezer and a femtosecond laser tweezer. The difference of the optical trapping force between CW and femtosecond optical tweezers increased as the particle size decreased, possibly due to the self-focusing effect of the ultrashort pulses. Also, the white damage spots were generated near the focus during femtosecond optical trapping of biological cells even with extremely low average power. The instantaneous optical damage threshold was measured as a function of the trap depth as well. These results may help to optimize the optical trapping of biological cells using femtosecond lasers.

Paper Details

Date Published: 29 March 2005
PDF: 7 pages
Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); doi: 10.1117/12.591446
Show Author Affiliations
Kang-Bin Im, Yonsei Univ. (South Korea)
Sumin Han, Yonsei Univ. (South Korea)
Hwajoon Park, Yonsei Univ. (South Korea)
Dan Jin, Yonsei Univ. (South Korea)
Soo-Kie Kim, Yonsei Univ. (South Korea)
Beop-Min Kim, Yonsei Univ. (South Korea)


Published in SPIE Proceedings Vol. 5699:
Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III
Dan V. Nicolau; Dan V. Nicolau; Jörg Enderlein; Ramesh Raghavachari; Robert C. Leif; Daniel L. Farkas, Editor(s)

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