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Proceedings Paper

SERS quantitative urine creatinine measurement of human subject
Author(s): Tsuei Lian Wang; Hui-hua Kenny Chiang; Hui-hsin Lu; Yung-da Hung
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Paper Abstract

SERS method for biomolecular analysis has several potentials and advantages over traditional biochemical approaches, including less specimen contact, non-destructive to specimen, and multiple components analysis. Urine is an easily available body fluid for monitoring the metabolites and renal function of human body. We developed surface-enhanced Raman scattering (SERS) technique using 50nm size gold colloidal particles for quantitative human urine creatinine measurements. This paper shows that SERS shifts of creatinine (104mg/dl) in artificial urine is from 1400cm-1 to 1500cm-1 which was analyzed for quantitative creatinine measurement. Ten human urine samples were obtained from ten healthy persons and analyzed by the SERS technique. Partial least square cross-validation (PLSCV) method was utilized to obtain the estimated creatinine concentration in clinically relevant (55.9mg/dl to 208mg/dl) concentration range. The root-mean square error of cross validation (RMSECV) is 26.1mg/dl. This research demonstrates the feasibility of using SERS for human subject urine creatinine detection, and establishes the SERS platform technique for bodily fluids measurement.

Paper Details

Date Published: 31 March 2005
PDF: 8 pages
Proc. SPIE 5703, Plasmonics in Biology and Medicine II, (31 March 2005); doi: 10.1117/12.591393
Show Author Affiliations
Tsuei Lian Wang, National Yang-Ming Univ. (Taiwan)
Hui-hua Kenny Chiang, National Yang-Ming Univ. (Taiwan)
Hui-hsin Lu, National Yang-Ming Univ. (Taiwan)
Yung-da Hung, National Yang-Ming Univ. (Taiwan)


Published in SPIE Proceedings Vol. 5703:
Plasmonics in Biology and Medicine II
Tuan Vo-Dinh; Joseph R. Lakowicz; Zygmunt K. Gryczynski, Editor(s)

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