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Proceedings Paper

Chemiluminescence and fluorescence spectrum methods for determination of Aflatoxin B1 mediated by FCLA + BSA
Author(s): WenLi Chen; Da Xing
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Paper Abstract

BSA (Bovine Serum Albumin) can enlarge the CL intensity of FCLA(3,7-dihydro-6-{4-{2-(N'-(5-fluoresceinyl) thioureido)ethoxy}phenyl}-2-methylimi-dazo{1,2-a}pyrazin-3-one dosium salt) to 763%. This report presents novel methods for determination of Aflatoxin B1 (AfB1) mediated by FCLA+BSA. The concentration of AFB1 showed an obvious positive correlation with the chemiluminescence (CL) intensity mediated by FCLA+BSA, correlative coefficient R@0.94. This method could measure accurately ng/ml of AfB1 concentration. 365nm as excitated wavelength, 440nm and 520nm-two fluorescence peaks of FCLA+BSA+AfB1 were found. The fluorescence intensity of peak at 440nm showed an obvious positive correlation with the concentration of AFB1, R@0.97; the fluorescence intensity of peak at 520nm showed a positive correlation with the concentration of AFB1, R@0.90. Comparing the peak of FCLA, FCLA+BSA and FCLA+BSA+AfB1 had a 6nm Einstein shift (red shift). The study suggested that CL and fluorescence spectrum methods mediated by FCLA+BSA might be applicable to the determination of AfB1 concentration.

Paper Details

Date Published: 4 April 2005
PDF: 8 pages
Proc. SPIE 5704, Genetically Engineered and Optical Probes for Biomedical Applications III, (4 April 2005); doi: 10.1117/12.591370
Show Author Affiliations
WenLi Chen, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)


Published in SPIE Proceedings Vol. 5704:
Genetically Engineered and Optical Probes for Biomedical Applications III
Darryl J. Bornhop; Samuel I. Achilefu; Ramesh Raghavachari; Alexander P. Savitsky, Editor(s)

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