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Proceedings Paper

In vitro NIR laser tissue welding of porcine ocular tissues
Author(s): Richard B. Rosen; Howard E. Savage; Rabindra K. Halder; Uladzimir Kartazayeu; Steven A. McCormick; Alvin Katz; Henry D. Perry; Robert R. Alfano
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Paper Abstract

In this study, 72 different combinations of laser welding parameters were compared for their effectiveness in welding ocular tissue. The laser employed in the welding system was a near infrared (NIR) erbium fiber laser with a wavelength of 1.455 μm . The laser system used a motorized translational stage and shutter to control the laser exposure of the tissue being welded. The emission wavelength of the laser in the NIR range corresponds to one of the lesser absorption bands of water. Parameters of the laser welding system that could be changed to allow a more effective distribution of the laser energy and therefore management of thermal energy included: the number and kinds of intricate offset patterns of light on or around the incision, the number of lines per pattern, the power level, the speed of the laser beam movement over the tissues, the spot size, dwell time and the focus plane of the light beam in the tissue. Histopathology was used as an endpoint indication of the effects that the various sets of welding parameters had on the welded tissues. Standard Hematoxylin and Eosin stain and Sirius Red F3B (Direct Red 80) in combination with polarization microscopy were used to stain and visualize the welded ocular tissue. Paradoxically, the best cornea welds quantified using histopathology occurred with fluence of 4,500 mJ/cm2 or less while the corneal welds exhibiting the strongest tensile strengths, but most tissue damage had a delivered fluence above 7,000 mJ/cm2. The best histological representatives of welded corneas had an average delivered fluence of 2,687 mJ/cm2 and an irradiance of 14 W/cm2. Using the properly determined parameters, the NIR erbium fiber welding system provided full thickness welds without the requirement of extrinsic dyes, chromophores, or solders. The NIR laser system with the appropriately developed parameters can be used effectively to weld ocular tissues.

Paper Details

Date Published: 25 April 2005
PDF: 5 pages
Proc. SPIE 5686, Photonic Therapeutics and Diagnostics, (25 April 2005); doi: 10.1117/12.591361
Show Author Affiliations
Richard B. Rosen, New York Eye and Ear Infirmary (United States)
Howard E. Savage, New York Eye and Ear Infirmary (United States)
Rabindra K. Halder, The City College of The City University of New York (United States)
Uladzimir Kartazayeu, The City College of The City University of New York (United States)
Steven A. McCormick, New York Eye and Ear Infirmary (United States)
Alvin Katz, The City College of The City University of New York (United States)
Henry D. Perry, New York Eye and Ear Infirmary (United States)
Robert R. Alfano, The City College of The City University of New York (United States)


Published in SPIE Proceedings Vol. 5686:
Photonic Therapeutics and Diagnostics
Brian Jet-Fei Wong; Eugene A. Trowers; Kenton W. Gregory; Abraham Katzir; Nikiforos Kollias; Reza S. Malek; Henry Hirschberg; Kenneth Eugene Bartels; Steen J. Madsen; Lloyd P. Tate; Lawrence S. Bass; Werner T. W. de Riese; Karen M. McNally-Heintzelman, Editor(s)

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