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Proceedings Paper

CARS microscopy studies of collagen transformation
Author(s): Georgi I. Petrov; L. Sona; Vladislav I. Shcheslavskiy; Vladislav V. Yakovlev
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Paper Abstract

Nonlinear Raman spectroscopy is employed to study structural transformation of collagen (type I) in solution. Relatively strong signal and polarization background suppression reveal significant changes of the vibrational spectrum of collagen, which are attributed to the formation of cholesteric globules. Circular dichroism spectroscopy confirms the concentration dependent structural transformation. Excellent axial resolution achieved in nonlinear Raman microscopy also allows to study for the first time the effect of large molecule adhesion on the surface.

Paper Details

Date Published: 30 March 2005
PDF: 8 pages
Proc. SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V, (30 March 2005); doi: 10.1117/12.591319
Show Author Affiliations
Georgi I. Petrov, Univ. of Wisconsin/Milwaukee (United States)
L. Sona, Univ. of Wisconsin/Milwaukee (United States)
Vladislav I. Shcheslavskiy, Univ. of Southampton (United Kingdom)
Vladislav V. Yakovlev, Univ. of Wisconsin/Milwaukee (United States)


Published in SPIE Proceedings Vol. 5700:
Multiphoton Microscopy in the Biomedical Sciences V
Ammasi Periasamy; Peter T. C. So, Editor(s)

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