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Proceedings Paper

Real-time imaging of membrane potentials during exposure to nanosecond pulsed electric fields
Author(s): Juergen F. Kolb; Wolfgang Frey; Jody A. White; R. O. Price; Peter F. Blackmore; Stephen J. Beebe; Ravindra P. Joshi; Karl H. Schoenbach
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Paper Abstract

The charging of mammalian cell plasma membranes in response to ultrashort pulsed electric fields of 60 ns and field strengths up to 100 kV/cm was investigated. Membranes of Jurkat cells were stained with a potential-sensitive dye, Annine-6 and placed in a microreactor mounted on an inverted fluorescence microscope. Images of changes in the fluorescence intensity during the exposure were recorded with a high-sensitivity CCD-camera. A temporal resolution of 5 ns was achieved by illuminating the cells with a 5 ns laser pulse from a dye-laser. The laser pulse was synchronized with the high voltage pulse to record images at specific times before, during and after exposure to the electric field. When exposing Jurkat cells to a 60 ns, 100 kV/cm pulse, each hemisphere of the plasma membrane (as oriented with respect to the electrodes) responded uniquely to the applied field. From these observations it is possible to draw conclusions on the charging time of the membrane, maximum transmembrane voltages and the onset of poration.

Paper Details

Date Published: 1 April 2005
PDF: 7 pages
Proc. SPIE 5692, Advanced Biomedical and Clinical Diagnostic Systems III, (1 April 2005); doi: 10.1117/12.591313
Show Author Affiliations
Juergen F. Kolb, Ctr. for Bioelectrics, Old Dominion Univ. (United States)
Wolfgang Frey, Forschungszentrum Karlsruhe GmbH (Germany)
Jody A. White, Ctr. for Bioelectrics, Old Dominion Univ. (United States)
R. O. Price, Ctr. for Bioelectrics, Old Dominion Univ. (United States)
Peter F. Blackmore, Eastern Virginia Medical School (United States)
Stephen J. Beebe, Eastern Virginia Medical School (United States)
Ravindra P. Joshi, Old Dominion Univ. (United States)
Karl H. Schoenbach, Ctr. for Bioelectrics, Old Dominion Univ. (United States)
Old Dominion Univ. (United States)


Published in SPIE Proceedings Vol. 5692:
Advanced Biomedical and Clinical Diagnostic Systems III
Tuan Vo-Dinh; Warren S. Grundfest; David A. Benaron; Gerald E. Cohn, Editor(s)

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