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Proceedings Paper

AOTF-based system for image cytometry
Author(s): Bartek Rajwa; Wamiq Ahmed; Murugesan Venkatapathi; Gerald Gregori; Feng Jin; Jolanta Soos; Sudhir Trivedi; J. Paul Robinson
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Paper Abstract

This paper describes a simple, inexpensive multispectral imaging system for image cytometry applications. The system is based on an acousto-optical tunable filter (AOTF), a monochrome CCD camera, and a research-grade fluorescence microscope. The selected TeO2 AOTF has a 10x10 mm2 entrance aperture and operates within the spectral range of 447-750 nm. The bandpass of the filter varies between 1.4 nm at 450 nm and 5.1 nm at 690 nm. The control software works within the environment of a popular image-acquisition and -processing package Image Pro-Plus, making this system easy to integrate with many existing fluorescence microscopes and cameras. Since image-cytometry applications do not require very high spatial resolution, AOTF-based systems may become an interesting alternative to more complex and expensive LCTF or pushbroom methods.

Paper Details

Date Published: 23 March 2005
PDF: 8 pages
Proc. SPIE 5694, Spectral Imaging: Instrumentation, Applications, and Analysis III, (23 March 2005); doi: 10.1117/12.591115
Show Author Affiliations
Bartek Rajwa, Purdue Univ. Cytometry Labs. (United States)
Wamiq Ahmed, Purdue Univ. Cytometry Labs. (United States)
Murugesan Venkatapathi, Purdue Univ. Cytometry Labs. (United States)
Gerald Gregori, Purdue Univ. Cytometry Labs. (United States)
Feng Jin, Brimrose Corp. of America (United States)
Jolanta Soos, Brimrose Corp. of America (United States)
Sudhir Trivedi, Brimrose Corp. of America (United States)
J. Paul Robinson, Purdue Univ. Cytometry Labs. (United States)


Published in SPIE Proceedings Vol. 5694:
Spectral Imaging: Instrumentation, Applications, and Analysis III
Gregory H. Bearman; Anita Mahadevan-Jansen; Richard M. Levenson, Editor(s)

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