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Proceedings Paper

Hyper-Rayleigh scattering as a method for probing collagen transformations in solutions
Author(s): Vladislav I. Shcheslavskiy; Georgi I. Petrov; Vladislav V. Yakovlev
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Paper Abstract

Hyper-Rayleigh scattering is used to measure the nonlinear optical properties of collagen type I in acetic acid solution. We find that the first hyperpolarizability unexpectedly drops with the increase of the concentration of collagen in solution. Circular dichroism absorption spectroscopy and UV absorption measurements indicate that the helical conformation remains stable, and the changes of nonlinear optical properties of collagen molecules are, most probably, due to the formation of some supramolelcular structures of collagen in solution.

Paper Details

Date Published: 29 March 2005
PDF: 9 pages
Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); doi: 10.1117/12.591094
Show Author Affiliations
Vladislav I. Shcheslavskiy, Univ. of Wisconsin/Milwaukee (United States)
Georgi I. Petrov, Univ. of Wisconsin/Milwaukee (United States)
Vladislav V. Yakovlev, Univ. of Wisconsin/Milwaukee (United States)


Published in SPIE Proceedings Vol. 5699:
Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III
Dan V. Nicolau; Dan V. Nicolau; Jörg Enderlein; Ramesh Raghavachari; Robert C. Leif; Daniel L. Farkas, Editor(s)

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