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Proceedings Paper

Optical monitoring of thermal effects in the retina during laser exposure
Author(s): Georg Schuele; Philip Huie; Dimitri Yellachich; Fanni Molnar; Caitlin O’Conell-Rodwell; Edward Vitkin; Lev Perelman; Daniel Palanker
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Paper Abstract

Fast and non-invasive detection of cellular stress is useful for fundamental research and practical applications in medicine and biology. Using Light Scattering Spectroscopy we extract information about changes in refractive index and size of the cellular organelles. Particle sizes down to 50nm in diameter can be detected using light within the spectral range of 450-850 nm. We monitor the heat-induced sub-cellular structural changes in human RPE cells and, for comparison, in transfected NIH-3T3 cells which express luciferase linked to the heat shock protein (HSP). Using inverse light scattering fitting algorithm, we reconstruct the size distribution of the sub-micron organelles from the light scattering spectrum. The most significant (up to 70%) and rapid (20sec) temperature-related changes can be linked to an increase of refractive index of the 160nm sized mitochondria. The start of this effect coincides with the onset of HSP expression. This technique provides an insight into metabolic processes within organelles larger than 50nm without exogenous staining and opens doors for non-invasive real-time assessment of cellular stress, which can be used for monitoring of retinal laser treatments like transpupillary thermo therapy or PDT.

Paper Details

Date Published: 18 April 2005
PDF: 7 pages
Proc. SPIE 5688, Ophthalmic Technologies XV, (18 April 2005); doi: 10.1117/12.591068
Show Author Affiliations
Georg Schuele, Stanford Univ. (United States)
Philip Huie, Stanford Univ. (United States)
Dimitri Yellachich, Stanford Univ. (United States)
Fanni Molnar, Stanford Univ. (United States)
Caitlin O’Conell-Rodwell, Stanford Univ. (United States)
Edward Vitkin, Harvard Univ. (United States)
Lev Perelman, Harvard Univ. (United States)
Daniel Palanker, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 5688:
Ophthalmic Technologies XV
Fabrice Manns; Bruce E. Stuck; Michael Belkin; Per G. Söderberg; Arthur Ho, Editor(s)

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