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Proceedings Paper

Are luminescent quantum dots efficient energy acceptors?
Author(s): Aaron R. Clapp; Igor L. Medintz; Brent R. Fisher; Hedi Mattoussi
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Paper Abstract

Steady state and time-resolved fluorescence measurements were used to investigate the ability of luminescent quantum dots (QDs) to function as efficient energy acceptors in fluorescence resonance energy transfer (FRET) binding assays with organic dye donors. Fluorescent dyes, AlexaFluor 488 or Cy3, were used with various QD acceptors in QD-dye-labeled-protein conjugates. Data derived from both sets of experiments showed no apparent FRET from dye to QD. The collected data were discussed within the framework of a competition between a fast radiative decay rate of the donor excitation and a slower FRET decay rate. This is due to the long exciton lifetime of the acceptor compared to that of the dye, combined with substantial QD direct excitation.

Paper Details

Date Published: 28 April 2005
PDF: 9 pages
Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); doi: 10.1117/12.590989
Show Author Affiliations
Aaron R. Clapp, Naval Research Lab. (United States)
Igor L. Medintz, Naval Research Lab. (United States)
Brent R. Fisher, Massachusetts Institute of Technology (United States)
Hedi Mattoussi, Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 5705:
Nanobiophotonics and Biomedical Applications II
Alexander N. Cartwright; Marek Osinski, Editor(s)

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