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Proceedings Paper

Direct led-fluorescence method for Mao-B inactivation in the treatment of Parkinson's
Author(s): Jimmy A. Castillo; Jannett Hung; M. Rodriguez; E. Bastidas; I. Laboren; A. Jaimes
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Paper Abstract

A led-fluorescence spectroscopy method determinate the inhibitory effects of probe compounds on MAO-B activity is described. In this assay, we demonstrate the possibility of determinate the activity of MAO-B efficiently and rapidly without the use of reference substrate. Measuring variations in fluorescence intensity of MAO-B enzyme during the reaction with inhibitors, L-deprenyl and berberine IC50 and KI values were obtained. For L-deprenyl (IC50 = 0.017 μM and KI = 0.019 μM) and berberine (IC50 = 90 μM and KI = 47 μM) were in agreement to the values obtained with a standard method and literature reported.

Paper Details

Date Published: 21 October 2004
PDF: 6 pages
Proc. SPIE 5622, 5th Iberoamerican Meeting on Optics and 8th Latin American Meeting on Optics, Lasers, and Their Applications, (21 October 2004); doi: 10.1117/12.590947
Show Author Affiliations
Jimmy A. Castillo, Univ. Central de Venezuela (Venezuela)
Jannett Hung, Instituto Venezolano de Investigaciones Cientificas (Venezuela)
M. Rodriguez, Univ. Central de Venezuela (Venezuela)
E. Bastidas, Univ. Central de Venezuela (Venezuela)
I. Laboren, Univ. Central de Venezuela (Venezuela)
A. Jaimes, Univ. Central de Venezuela (Venezuela)


Published in SPIE Proceedings Vol. 5622:
5th Iberoamerican Meeting on Optics and 8th Latin American Meeting on Optics, Lasers, and Their Applications

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