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Proceedings Paper

Synthesis and characterization of CdTe nanocrystals for applications as biolabels
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Paper Abstract

Semiconductor nanocrystals composed by few hundred to a few thousand atoms also known as quantum dots have received substantial attention due to their size tunable narrow-emission spectra and several other advantages over organic molecules as fluorescent labels for biological applications, including resistence to photodegradation, improved brightness and only one laser excitation that enable the monitoring of several processes simultaneously. In this work we have synthesized and characterized thiol-capped CdTe and bioconjugated them to macrophages. We have mapped the fluoroscence intensity along the macrophage's body in our set up consisting of an optical tweezer plus a non-linear micro-spectroscopy system to perform scanning microscopy and observe spectra using two photon excited luminescence.

Paper Details

Date Published: 4 April 2005
PDF: 7 pages
Proc. SPIE 5704, Genetically Engineered and Optical Probes for Biomedical Applications III, (4 April 2005); doi: 10.1117/12.590841
Show Author Affiliations
Wendel L. Moreira, Univ. Estadual de Campinas (Brazil)
Adriana Fontes, Univ. Estadual de Campinas (Brazil)
Andre Thomaz, Univ. Estadual de Campinas (Brazil)
Antonio A.R. Neves, Univ. Estadual de Campinas (Brazil)
Luiz Carlos Barbosa, Univ. Estadual de Campinas (Brazil)
Frederico D. de Menezes, Univ. Federal de Pernambuco (Brazil)
Patricia M. A. de Farias, Univ. Federal de Pernambuco (Brazil)
Beate S. Santos, Univ. Federal de Pernambuco (Brazil)
Carlos Lenz Cesar, Univ. Estadual de Campinas (Brazil)


Published in SPIE Proceedings Vol. 5704:
Genetically Engineered and Optical Probes for Biomedical Applications III
Darryl J. Bornhop; Samuel I. Achilefu; Ramesh Raghavachari; Alexander P. Savitsky, Editor(s)

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