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Proceedings Paper

Quantitative morphology of cytoskeletal organization: new classifier architectures and applications
Author(s): Giovanni Franco Crosta; Chiara Urani; Laura Fumarola; Rosa Valentina Chieppa
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Paper Abstract

Recently, these authors developed a heterogeneous, one-level image classifier (CH) based on morphological descriptors from direct domain analysis (spatial differentiation), fractal analysis and “spectrum enhancement” (a kind of non-linear filtering). Classifier CH was applied to epi-fluorescence microscope images of cytoskeletal microtubules and was trained to recognize structural alterations of the cytoskeleton in various circumstances. The application dealt with images of rat hepatocytes (rh). The scope of this paper is twofold: a) to investigate different classifier architectures, which include the multiobjective optimization of some image analysis parameters by means of suitable algorithms; b) to apply said classifiers to new sets of images obtained from mouse fibroblasts (mf) and HepG2 (hg) cells. Image sets from control and treated cell cultures are analyzed. Classifier CH is applied to mf microtubules. A new classifier entirely relying on “spectrum enhancement” (although on different descriptors) is developed and applied to rh and hg images. From the latter classifier, by bringing in descriptors from direct domain and fractal analysis, a hierarchical classifier is derived and applied to rh images. Results are compared. Classifier performance is expressed in terms of sensitivity, specificity and information contents of the first three principal components.

Paper Details

Date Published: 29 March 2005
PDF: 11 pages
Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); doi: 10.1117/12.590802
Show Author Affiliations
Giovanni Franco Crosta, Univ. degli Studi di Milano-Bicocca (Italy)
Chiara Urani, Univ. degli Studi di Milano-Bicocca (Italy)
Laura Fumarola, Univ. degli Studi di Milano-Bicocca (Italy)
Rosa Valentina Chieppa, Univ. degli Studi di Milano-Bicocca (Italy)

Published in SPIE Proceedings Vol. 5699:
Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III
Dan V. Nicolau; Dan V. Nicolau; Jörg Enderlein; Ramesh Raghavachari; Robert C. Leif; Daniel L. Farkas, Editor(s)

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