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Proceedings Paper

Unexpected long-term stability of a cyanine dye bioconjugate
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Paper Abstract

Biocompatible organic dyes, in general, exhibit poor photostability in aqueous solution. This is especially true at the high concentrations needed for bolus administration in a clinical application. Several years ago, we showed that by using carefully chosen macromolecular additives, the stability of aqueous dye solutions may be enhanced significantly. We now report the observation of an unexpected long-term stability of a near infrared dye-peptide conjugate. The Mallinckrodt developed dye-peptide conjugate known as Cytate is an indocyanine type dye attached to the peptide octreotate. We have previously shown that this compound targets somatostatin receptor rich tumors and provides unambiguous contrast for optical imaging. A freshly prepared aqueous solution of Cytate was mixed with blood (from a rat) in a small glass bottle. An optical image was taken soon after, along with several control samples. The Cytate-blood bottle fluoresced quite nicely. The sample bottles were then left in a lab for three plus years, at room temperature with ordinary exposure to lab lights. The sample bottles were imaged after the three year period, and the Cytate-blood bottle fluoresces still.

Paper Details

Date Published: 4 April 2005
PDF: 7 pages
Proc. SPIE 5704, Genetically Engineered and Optical Probes for Biomedical Applications III, (4 April 2005); doi: 10.1117/12.590506
Show Author Affiliations
Richard B. Dorshow, Mallinckrodt Inc. (United States)
Raghavan Rajagopalan, Mallinckrodt Inc. (United States)

Published in SPIE Proceedings Vol. 5704:
Genetically Engineered and Optical Probes for Biomedical Applications III
Darryl J. Bornhop; Samuel I. Achilefu; Ramesh Raghavachari; Alexander P. Savitsky, Editor(s)

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