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Proceedings Paper

Multiphoton nanosurgery in cells and tissues
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Paper Abstract

Multiphoton Microscopy with a femtosecond pulsed Ti:sapphire laser in the near infrared (NIR) enables the user not only to image cells and tissues with a subcellular resolution but also to perform highly precise nanosurgery. Intratissue compartments, single cells and even cell organelles like mitochondria, membranes or chromosomes can be manipulated and optically knocked out. Working at transient TW/cm2 laser intensities, single cells of tumor-sphaeroids were eliminated efficiently inside the sphaeroid without damaging the neighbour cells. Also single organelles of cells inside tissues could be optically knocked out with the nanoscalpel without collateral damage. Tissue structures inside a human tooth have been ablated with sizes below 1 μm. This method may become a useful instrument for nano-manipulating and surgery in several fields of science, including targeted transfection.

Paper Details

Date Published: 15 April 2005
PDF: 9 pages
Proc. SPIE 5695, Optical Interactions with Tissue and Cells XVI, (15 April 2005); doi: 10.1117/12.590487
Show Author Affiliations
Iris Riemann, Fraunhofer-Institut fuer Biomedizinische Technik (Germany)
Tiemo Anhut, Fraunhofer-Institut fuer Biomedizinische Technik (Germany)
Frank Stracke, Fraunhofer-Institut fuer Biomedizinische Technik (Germany)
Ronan Le Harzic, JenLab GmbH (Germany)
Karsten Koenig, Fraunhofer-Institut fuer Biomedizinische Technik (Germany)

Published in SPIE Proceedings Vol. 5695:
Optical Interactions with Tissue and Cells XVI
Steven L. Jacques; William P. Roach, Editor(s)

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