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Proceedings Paper

High-throughput scanning with single-molecule sensitivity
Author(s): Max Sonnleitner; Gunter Freudenthaler; Jan Hesse; Gerhard J. Schuetz
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Paper Abstract

We report on the application of a novel fluorescence-microscope based scanning device with single molecule sensitivity to microarray readout. The device is based on a CCD camera operated in time delay and integration (TDI) mode synchronized with the movement of a sample scanning stage, enabling continuous data acquisition. The implementation of a focus hold system keeps the sample in focus during scanning. Results from ultra-sensitive high-resolution microarray readout provide clear evidence for the superiority of the novel detection method over conventional microarray readout systems in particular regarding to sensitivity and dynamic range. Minute sample amount and lacking amplification methods will demand for this ultra-sensitive readout technology in future genomic and proteomic research.

Paper Details

Date Published: 29 March 2005
PDF: 9 pages
Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); doi: 10.1117/12.590292
Show Author Affiliations
Max Sonnleitner, Upper Austrian Research GmbH (Austria)
Gunter Freudenthaler, Upper Austrian Research GmbH (Austria)
Jan Hesse, Univ. of Linz (Austria)
Gerhard J. Schuetz, Univ. of Linz (Austria)


Published in SPIE Proceedings Vol. 5699:
Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III
Dan V. Nicolau; Dan V. Nicolau; Jörg Enderlein; Ramesh Raghavachari; Robert C. Leif; Daniel L. Farkas, Editor(s)

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