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Proceedings Paper

Optical micromanipulation of mixed yeast cell populations for analyzing growth behavior
Author(s): Jesper Glueckstad; Peter J. Rodrigo; Vincent R. Daria; Henrik Siegumfeldt; Peter Nissen; Nils Arneborg
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Paper Abstract

We use spatially sculptured light for user-interactive micromanipulation of mixed yeast cell populations to analyze growth behavioural patterns. There is negligible absorption in the near-infrared region of the light spectrum making it suitable for direct manipulation of individual cells in a growing population. Rather than using a single-beam optical trap, multiple cells are manipulated using a system based on the Generalized Phase Contrast (GPC) method, which allows arbitrary trapping configurations i.e. control over the number of traps, and the size/shape of each trap. This enables the cells to be selectively trapped in all three-dimensions (3D) and manipulated in real-time while under direct observation. Here, we impose controlled experiments using these multiple 3D optical traps to show the alteration of growth patterns in mixed cultures of Saccharomyces cerevisiae and Hanseniaspora uvarum experiencing spatially constrained conditions.

Paper Details

Date Published: 29 March 2005
PDF: 7 pages
Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); doi: 10.1117/12.590264
Show Author Affiliations
Jesper Glueckstad, Riso National Lab. (Denmark)
Peter J. Rodrigo, Riso National Lab. (Denmark)
Vincent R. Daria, Riso National Lab. (Denmark)
Henrik Siegumfeldt, Royal Veterinary and Agricultural Univ. (Denmark)
Peter Nissen, Royal Veterinary and Agricultural Univ. (Denmark)
Nils Arneborg, Royal Veterinary and Agricultural Univ. (Denmark)


Published in SPIE Proceedings Vol. 5699:
Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III
Dan V. Nicolau; Ramesh Raghavachari; Dan V. Nicolau; Jörg Enderlein; Robert C. Leif; Daniel L. Farkas, Editor(s)

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