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Proceedings Paper

Cholesterol dependence of cell membrane dynamics
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Paper Abstract

Membranes of living cells are characterized by laser-assisted fluorescence microscopy, in particular a combination of microspectrofluorometry, total internal reflection fluorescence microscopy (TIRFM) and fluorescence decay kinetics. The generalized polarization (GP, characterizing a spectral shift which depends on the phase of membrane lipids), the time constant of fluorescence anisotropy (τr) as well as the fluorescence lifetime (τ) of the membrane marker laurdan revealed to be appropriate measures for membrane stiffness and fluidity. GP decreased with increasing temperature and was always higher for the plasma membrane than for intracellular membranes. The latter effect was correlated with the intracellular content of cholesterol, which could be modified using defined protocols of depletion or enrichment. Concomitant with generalized polarization the fluorescence lifetime τ increased with the content of cholesterol. Changes of cholesterol amounts in cell membranes have previously been related to specific diseases and may have some influence on the uptake of pharmaceutical agents.

Paper Details

Date Published: 29 March 2005
PDF: 7 pages
Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); doi: 10.1117/12.590236
Show Author Affiliations
Petra Weber, Hochschule Aalen (Germany)
Michael Wagner, Hochschule Aalen (Germany)
Reinhard Sailer, Univ. Ulm (Germany)
Wolfgang S.L. Strauss, Univ. Ulm (Germany)
Herbert Schneckenburger, Hochschule Aalen (Germany)
Univ. Ulm (Germany)


Published in SPIE Proceedings Vol. 5699:
Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III
Dan V. Nicolau; Dan V. Nicolau; Jörg Enderlein; Ramesh Raghavachari; Robert C. Leif; Daniel L. Farkas, Editor(s)

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