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Proceedings Paper

Three-dimensional analysis tool for segmenting and measuring the structure of telomeres in mammalian nuclei
Author(s): Bart J. Vermolen; Ian T. Young; Alice Chuang; Landon Wark; Tony Chuang; Sabine Mai; Yuval Garini
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Paper Abstract

Quantitative analysis in combination with fluorescence microscopy calls for innovative digital image measurement tools. We have developed a three-dimensional tool for segmenting and analyzing FISH stained telomeres in interphase nuclei. After deconvolution of the images, we segment the individual telomeres and measure a distribution parameter we call ρT. This parameter describes if the telomeres are distributed in a sphere-like volume (ρT ≈ 1) or in a disk-like volume (ρT >> 1). Because of the statistical nature of this parameter, we have to correct for the fact that we do not have an infinite number of telomeres to calculate this parameter. In this study we show a way to do this correction. After sorting mouse lymphocytes and calculating ρT and using the correction introduced in this paper we show a significant difference between nuclei in G2 and nuclei in either G0/G1 or S phase. The mean values of ρT for G0/G1, S and G2 are 1.03, 1.02 and 13 respectively.

Paper Details

Date Published: 29 March 2005
PDF: 10 pages
Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); doi: 10.1117/12.590232
Show Author Affiliations
Bart J. Vermolen, Delft Univ. of Technology (Netherlands)
Ian T. Young, Delft Univ. of Technology (Netherlands)
Alice Chuang, Univ. of Manitoba (Canada)
Landon Wark, Univ. of Manitoba (Canada)
Tony Chuang, Univ. of Manitoba (Canada)
Sabine Mai, Univ. of Manitoba (Canada)
Yuval Garini, Delft Univ. of Technology (Netherlands)

Published in SPIE Proceedings Vol. 5699:
Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III
Dan V. Nicolau; Dan V. Nicolau; Jörg Enderlein; Ramesh Raghavachari; Robert C. Leif; Daniel L. Farkas, Editor(s)

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