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Proceedings Paper

Photodynamic efficacy and spectroscopic properties of 5-ALA-induced protoporphyrin IX in human glioblastoma cells
Author(s): Reinhard Sailer; Wolfgang S.L. Strauss; Helmut Emmert; Michael Wagner; Rudolf Steiner; Herbert Schneckenburger
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Paper Abstract

One of the most promising clinical applications of 5-aminolevulinic acid (5-ALA) induced protoporphyrin IX (PP IX) is the fluorescence diagnosis and photodynamic treatment of malignant brain tumours. In order to obtain a deeper understanding of the cellular processes involved, U373-MG human glioblastoma cells were used as model system to study intracellular location, fluorescence properties and light-induced reactions of the photosensitizer. In comparison with ovarian cancer or breast cancer cell lines, glioblastoma cells accumulated PP IX only to a moderate extent, but were most effectively inactivated (highest photodynamic efficacy per photosensitizer molecule). In contrast to breast cancer cells, which showed a rather granular PP IX fluorescence pattern, PP IX fluorescence in U373-MG cells seemed to originate mainly from cellular membranes. In addition, photobleaching and lowering of fluorescence lifetimes during irradiation were comparably small for this cell line. Combining the results of fluorescence lifetime imaging microscopy (FLIM) and photobleaching measurements, we deduced that those cellular sites where PP IX fluorescence was most pronounced, contributed rather little to its photodynamic efficacy.

Paper Details

Date Published: 8 April 2005
PDF: 7 pages
Proc. SPIE 5689, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIV, (8 April 2005); doi: 10.1117/12.590120
Show Author Affiliations
Reinhard Sailer, Institut fur Lasertechnologien in der Medizin und Messtechnik/Univ. Ulm (Germany)
Wolfgang S.L. Strauss, Institut fur Lasertechnologien in der Medizin und Messtechnik/Univ. Ulm (Germany)
Helmut Emmert, Institut fur Lasertechnologien in der Medizin und Messtechnik/Univ. Ulm (Germany)
Michael Wagner, Hochschule Aalen (Germany)
Rudolf Steiner, Institut fur Lasertechnologien in der Medizin und Messtechnik/Univ. Ulm (Germany)
Herbert Schneckenburger, Institut fur Lasertechnologien in der Medizin und Messtechnik/Univ. Ulm (Germany)
Hochschule Aalen (Germany)


Published in SPIE Proceedings Vol. 5689:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIV
David Kessel, Editor(s)

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