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Proceedings Paper

Automated high-throughput micro-injection system for floating cells
Author(s): Satoru Sakai; Sachihiro Youoku; Yoshinori Suto; Moritoshi Ando; Akio Ito
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Paper Abstract

We have developed an automated microinjection system that captures many floating cells and controls capillary positions precisely. To capture many cells simultaneously, we constructed an array of holes on a 10 x 10 mm silicon-based substrate. The hole diameter is 3 μm because our target cells are 10 - 20 μm in diameter. A suction pump connected to the bottom of the multi-hole silicon chip draws the medium into the holes using a slight vacuum, so cells are caught there. Using an initial prototype chip having 121 holes, we captured over 90 cells in a single sweep. Automated microinjection requires precise control of the capillary positions, so images of the capillary and holes on the chip are observed using a microscope with a CCD camera located above the biological medium. The 3D positions of these elements are accurately measured by processing these images. The capillary and the chip are mounted on automatic stages individually controlled with this position data. Using these techniques, this system can microinject about one cell per second. Its success rate for microinjection is 61% for PC12 cells.

Paper Details

Date Published: 29 March 2005
PDF: 8 pages
Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); doi: 10.1117/12.590014
Show Author Affiliations
Satoru Sakai, Fujitsu Ltd. (Japan)
Sachihiro Youoku, Fujitsu Ltd. (Japan)
Yoshinori Suto, Fujitsu Ltd. (Japan)
Moritoshi Ando, Fujitsu Ltd. (Japan)
Akio Ito, Fujitsu Ltd. (Japan)

Published in SPIE Proceedings Vol. 5699:
Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III
Dan V. Nicolau; Dan V. Nicolau; Jörg Enderlein; Ramesh Raghavachari; Robert C. Leif; Daniel L. Farkas, Editor(s)

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