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Proceedings Paper

Pulsed photodynamic inactivation of gram-negative bacteria: electron microscopic study
Author(s): Masafumi Nagasawa; Shunichi Sato; Satoko Kawauchi; Nariyoshi Shinomiya; Daizo Saito; Hiroshi Ashida; Minoru Obara
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Paper Abstract

We previously demonstrated the methylene blue-mediated photodynamic inactivation of P. aeruginosa in vitro using pulsed light excitation. However, the photobactericidal mechanism has not been revealed yet. In this study, morphological changes induced by pulsed photodynamic treatment in bacteria were investigated by electron microscopy. The micrographs of bacteria showed an alteration in the membrane structure in the early period of illumination, and increase in the total light dose caused an alteration in the cytoplasmic structure. It was shown that the membrane damage may be lethal to bacteria.

Paper Details

Date Published: 8 April 2005
PDF: 6 pages
Proc. SPIE 5689, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIV, (8 April 2005); doi: 10.1117/12.589993
Show Author Affiliations
Masafumi Nagasawa, Keio Univ. (Japan)
Shunichi Sato, National Defense Medical College Research Institute (Japan)
Satoko Kawauchi, National Defense Medical College Research Institute (Japan)
Nariyoshi Shinomiya, National Defense Medical College Research Institute (Japan)
Daizo Saito, National Defense Medical College (Japan)
Hiroshi Ashida, National Defense Medical College Research Institute (Japan)
Minoru Obara, Keio Univ. (Japan)


Published in SPIE Proceedings Vol. 5689:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIV
David Kessel, Editor(s)

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