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Proceedings Paper

Study pollen grains in rice by using multispectral imaging techniques
Author(s): Qiongshui Wu; Yaojun Hu; Hengyu Ke; Libo Zeng; Yi Ding
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Paper Abstract

This paper describes a novel multispectral imaging microscope and its applications in the study of pollen grains in rice. The Imaging instruments can simultaneously record both spectral and spatial information of a sample, which is helpful to study the chemical states and physical properties of the sample by taking advantage of spatial image processing and spectroscopic analysis techniques. A LCTF (liquid crystal tunable filter) device is used for fast wavelength selection in the range of 400nm to 720nm and a cooled two-dimensional monochrome CCD for image detection. In this paper, the image acquisition process, spatial and spectral calibration and spectral imaging analysis methods are detailed. And also a novel method using this multispectral imaging microscope to observe rice pollen grains is reported here. The multispectral images were systematically processed and analyzed by the software. The results illustrated that the transmittance analysis of multispectral pollen images can accurately identify the pollen abortion stage of male-sterile rice, and can easily distinguish a variety of male sterile cytoplasm. Compared with cytological and histochemical methods reported previously, the method reported here has demonstrated to be more efficient and reliable in the study of chemical states and physical properties in plant cells.

Paper Details

Date Published: 23 March 2005
PDF: 10 pages
Proc. SPIE 5694, Spectral Imaging: Instrumentation, Applications, and Analysis III, (23 March 2005); doi: 10.1117/12.589589
Show Author Affiliations
Qiongshui Wu, Wuhan Univ. (China)
Yaojun Hu, Key Lab. MOE, Wuhan Univ. (China)
Hengyu Ke, Wuhan Univ. (China)
Libo Zeng, Wuhan Univ. (China)
Yi Ding, Key Lab. MOE, Wuhan Univ. (China)

Published in SPIE Proceedings Vol. 5694:
Spectral Imaging: Instrumentation, Applications, and Analysis III
Gregory H. Bearman; Anita Mahadevan-Jansen; Richard M. Levenson, Editor(s)

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