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Proceedings Paper

Peptide-coated semiconductor nanocrystals for biomedical applications
Author(s): Xavier Michalet; Fabien F. Pinaud; Laurent A. Bentolila; James M. Tsay; Soeren Doose; Jianqing J. Li; G. Iyer; Shimon Weiss
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Paper Abstract

We have developed a new functionalization approach for semiconductor nanocrystals based on a single-step exchange of surface ligands with custom-designed peptides. This peptide-coating technique yield small, monodisperse and very stable water-soluble NCs that remain bright and photostable. We have used this approach on several types of core and core-shell NCs in the visible and near-infrared spectrum range and used fluorescence correlation spectroscopy for rapid assessment of the colloidal and photophysical properties of the resulting particles. This peptide coating strategy has several advantages: it yields probes that are immediately biocompatible; it is amenable to improvements of the different properties (solubilization, functionalization, etc) via rational design, parallel synthesis, or molecular evolution; it permits the combination of several functions on individual NCs. These functionalized NCs have been used for diverse biomedical applications. Two are discussed here: single-particle tracking of membrane receptor in live cells and combined fluorescence and PET imaging of targeted delivery in live animals.

Paper Details

Date Published: 4 April 2005
PDF: 12 pages
Proc. SPIE 5704, Genetically Engineered and Optical Probes for Biomedical Applications III, (4 April 2005); doi: 10.1117/12.589498
Show Author Affiliations
Xavier Michalet, Univ. of California/Los Angeles (United States)
Fabien F. Pinaud, Univ. of California/Los Angeles (United States)
Laurent A. Bentolila, Univ. of California/Los Angeles (United States)
James M. Tsay, Univ. of California/Los Angeles (United States)
Soeren Doose, Univ. of Bielefeld (Germany)
Jianqing J. Li, Univ. of California/Los Angeles (United States)
G. Iyer, Univ. of California/Los Angeles (United States)
Shimon Weiss, Univ. of California/Los Angeles (United States)


Published in SPIE Proceedings Vol. 5704:
Genetically Engineered and Optical Probes for Biomedical Applications III
Darryl J. Bornhop; Samuel I. Achilefu; Ramesh Raghavachari; Alexander P. Savitsky, Editor(s)

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