Share Email Print
cover

Proceedings Paper

Advances in the processing of policromat images as diagnostic method to determine white spot syndrome virus in white shrimp (Litopenaeus vannamei)
Author(s): Cristina M. Chavez-Sanchez; Josue Alvarez-Borrego; L. Montoya-Rodriguez; A. Garcia-Gasca; Emma Josefina Fajer Avila; R. Pacheco-Marges
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

White spot syndrome (WSSV) is a viral disease which affects many crustacean species including commercial shrimps. Adequate, precise and quick methods to diagnose on time the presence of the disease in order to apply different strategies to avoid the dispersion and to reduce mortalities is necessary. Histopathology is an important diagnostic method. However, histopathology has the problem that requires time to prepare the histological slides and time to arrive to some diagnosis because this depend on the nature of the tissues, the pathogen(s) to find, the number of organisms, number of slides to analyze and the skill of the technician. This paper try to demonstrate the sensibility of one digital system of processing and recognition of images using color correlation with phase filters, to identify inclusion bodies of WSSV. Infected tissues were processed to obtain histological slides and to verify that the inclusion bodies observed were of WSV, in situ hybridization were carried out. The sensibility results of the recognition of the inclusion bodies of WSSV with the color correlation program was 86.1%. The highest percentage of recognition was in nervous system and tegument glands with 100%. The values in the stomach epithelium and heart tissue was 78.45% of recognition. Tissues with the lowest recognition values were lymphoid organ and hematopoietic tissue. It is necessary further studies to increase the sensibility and to obtain the specificity.

Paper Details

Date Published: 21 October 2004
PDF: 6 pages
Proc. SPIE 5622, 5th Iberoamerican Meeting on Optics and 8th Latin American Meeting on Optics, Lasers, and Their Applications, (21 October 2004); doi: 10.1117/12.589477
Show Author Affiliations
Cristina M. Chavez-Sanchez, Unidad Mazatlan en Acuicultura y Manejo Ambiental, CIAD (Mexico)
Josue Alvarez-Borrego, Ctr. de Investigacion Cientifica y de Educacion Superior de Ensenada (Mexico)
L. Montoya-Rodriguez, Unidad Mazatlan en Acuicultura y Manejo Ambiental, CIAD (Mexico)
A. Garcia-Gasca, Unidad Mazatlan en Acuicultura y Manejo Ambiental, CIAD (Mexico)
Emma Josefina Fajer Avila, Unidad Mazatlan en Acuicultura y Manejo Ambiental, CIAD (Mexico)
R. Pacheco-Marges, Unidad Mazatlan en Acuicultura y Manejo Ambiental, CIAD (Mexico)


Published in SPIE Proceedings Vol. 5622:
5th Iberoamerican Meeting on Optics and 8th Latin American Meeting on Optics, Lasers, and Their Applications
Aristides Marcano O.; Jose Luis Paz, Editor(s)

© SPIE. Terms of Use
Back to Top