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Proceedings Paper

Evaluation of vascular effects after photodynamic and photothermal therapies using benzoporphyrin derivative monoacid ring A on a rodent dorsal skinfold model
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Paper Abstract

Background and Objectives: Pulsed dye laser (PDL) irradiation is the standard clinical treatment for vascular lesions. However, PDL treatment of port wine stain birthmarks (PWS) is variable and unpredictable. Photodynamic therapy (PDT) using benzoporphyrin derivative monoacid ring A (BPD) and yellow light may induce substantial vascular effects and potentially offer a more effective treatment. In this study, we utilize a rodent dorsal skinfold model to evaluate the vascular effects of BPD-PDT at 576 nm as compared to PDL. Study Design/Materials and Methods: A dorsal skinfold window was created on the backs of female Sprague-Dawley rats, allowing epidermal and subdermal irradiation and subdermal imaging. One mg/kg BPD was administered intravenously via a jugular venous catheter. Study groups were: control (no BPD, no light), PDL (585 nm, τp 1.5 ms, 10 J/cm2), and PDT (BPD + continuous wave irradiation (CW) at 576nm, τp 16 min, 96 J/cm2). Vessels were imaged and assessed for damage using laser speckle imaging (LSI) before, immediately after, and 18 hours post-intervention. Results: Epidermal irradiation was accomplished without blistering, scabbing or ulceration. PDL and PDT resulted in similar reductions in vascular perfusion 18 hours post-intervention (34.6% and 33.4%, respectively). Conclusions: BPD-PDT can achieve safe and selective vascular effects and may offer an alternative therapeutic option for treatment of hypervascular skin lesions including PWS birthmarks.

Paper Details

Date Published: 25 April 2005
PDF: 8 pages
Proc. SPIE 5686, Photonic Therapeutics and Diagnostics, (25 April 2005); doi: 10.1117/12.589466
Show Author Affiliations
Tia K. Smith, Beckman Laser Institute and Medical Clinic, Univ. of California/Irvine (United States)
Bernard Choi, Beckman Laser Institute and Medical Clinic, Univ. of California/Irvine (United States)
Julio C. Ramirez-San-Juan, Beckman Laser Institute and Medical Clinic, Univ. of California/Irvine (United States)
Instituto Nacional de Astrofisica, Optica y Electronica (Mexico)
John Stuart Nelson, Beckman Laser Institute and Medical Clinic, Univ. of California/Irvine (United States)
Kristen M. Kelly, Beckman Laser Institute and Medical Clinic, Univ. of California/Irvine (United States)


Published in SPIE Proceedings Vol. 5686:
Photonic Therapeutics and Diagnostics
Brian Jet-Fei Wong; Eugene A. Trowers; Werner T. W. de Riese; Kenton W. Gregory; Abraham Katzir; Lawrence S. Bass; Karen M. McNally-Heintzelman; Nikiforos Kollias; Reza S. Malek; Henry Hirschberg; Steen J. Madsen; Kenneth Eugene Bartels; Lloyd P. Tate, Editor(s)

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