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Proceedings Paper

In vivo analysis of Drosophila embryo developmental dynamics by femtosecond pulse-induced ablation and multimodal nonlinear microscopy
Author(s): Willy Supatto; Delphine Debarre; Bruno Moulia; Eric Brouzes; Jean-Louis Martin; Marie-Claire Schanne-Klein; Emmanuel Farge; Emmanuel Beaurepaire
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Paper Abstract

Animal embryo development exhibits a complex ensemble of cell movements that are tightly regulated by developmental gene expression. It was proposed recently that mechanical factors may also play an important role during development. Investigating these dynamical processes is technically challenging and requires novel in vivo investigation methods. We show that multiphoton microscopy can be used for both perturbing and analyzing morphogenetic movements in vivo. (i) nonlinear microscopy is well adapted for the sustained imaging of early Drosophila embryos despite their highly scattering nature; (ii) femtosecond pulse-induced ablation can be used to process specific tissues in vivo. Combining this approach with multimodal microscopy (two-photon-excited fluorescence (2PEF) and third-harmonic generation (THG)), we report the successful quantitative modulation of morphogenetic movements in vivo. Our data provides insight to the issue of morphogenesis regulation.

Paper Details

Date Published: 30 March 2005
PDF: 9 pages
Proc. SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V, (30 March 2005); doi: 10.1117/12.589425
Show Author Affiliations
Willy Supatto, Institut Curie, CNRS (France)
Delphine Debarre, INSERM-CNRS, Ecole Polytechnique (France)
Bruno Moulia, Institut National de la Recherche Agronomique (France)
Eric Brouzes, Institut Curie, CNRS (France)
Jean-Louis Martin, INSERM-CNRS, Ecole Polytechnique (France)
Marie-Claire Schanne-Klein, INSERM-CNRS, Ecole Polytechnique (France)
Emmanuel Farge, Institut Curie, CNRS (France)
Emmanuel Beaurepaire, INSERM-CNRS, Ecole Polytechnique (France)


Published in SPIE Proceedings Vol. 5700:
Multiphoton Microscopy in the Biomedical Sciences V
Ammasi Periasamy; Peter T. C. So, Editor(s)

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