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Proceedings Paper

Fluorescence lifetime images and correlation spectra obtained by multidimensional TCSPC
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Paper Abstract

Multi-dimensional time-correlated single photon counting (TCSPC) is based on the excitation of the sample by a high-repetition rate laser and the detection of single photons of the fluorescence signal in several detection channels. Each photon is characterised by its time in the laser period, its detection channel number, and several additional variables such as the coordinates of an image area, or the time from the start of the experiment. Combined with a confocal or two-photon laser scanning microscope and a pulsed laser, multi-dimensional TCSPC makes a fluorescence lifetime technique with multi-wavelength capability, near-ideal counting efficiency, and the capability to resolve multi-exponential decay functions. We show that the same technique and the same hardware can be used to for precision fluorescence decay analysis, fluorescence correlation spectroscopy (FCS), and fluorescence intensity distribution analysis (FIDA and FILDA) in selected spots of a sample.

Paper Details

Date Published: 30 March 2005
PDF: 8 pages
Proc. SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V, (30 March 2005); doi: 10.1117/12.588990
Show Author Affiliations
Wolfgang Becker, Becker & Hickl GmbH (Germany)
Axel Bergmann, Becker & Hickl GmbH (Germany)
Elke Haustein, Technical Univ. Dresden (Germany)
Zdenek Petrasek, Technical Univ. Dresden (Germany)
Petra Schwille, Technical Univ. Dresden (Germany)
Christoph U. Biskup, Friedrich Schiller Univ. (Germany)
Tiemo Anhut, Fraunhofer Institute for Biomedical Engineering (Germany)
Iris Riemann, Fraunhofer Institute for Biomedical Engineering (Germany)
Karsten Konig, Fraunhofer Institute for Biomedical Engineering (Germany)


Published in SPIE Proceedings Vol. 5700:
Multiphoton Microscopy in the Biomedical Sciences V
Ammasi Periasamy; Peter T. C. So, Editor(s)

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