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Proceedings Paper

Dynamic detection of caspase-3 activation during photosensitization by fluorescence resonance energy transfer
Author(s): Yunxia Wu; Da Xing; Qun Chen; Tongsheng Chen; Yonghong Tang; Qingling Wan
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Paper Abstract

Apoptosis is one of the important modes in PDT-induced cell death. Activation of caspase-3 is considered to be the final step in many apoptosis pathways. In this study, we used SCAT3, a fluorescence resonance energy transfer (FRET) probe containing caspase-3 substrate, to study the dynamics of caspase-3 activation in living ASTC-a-1 cells expressing stably SCAT3. The FRET analysis results indicated that caspase-3 activation in response to tumor necrosis factor-α or PDT resulted in cleavage of the linker peptide and subsequent disruption of the FRET signal. The SCAT3 was cleaved immediately after PDT treatment, but that for TNF-a treatment was delayed two hours. Our experimental results suggested that the different apoptotic pathways induced by TNF-α or PDT caused different cleavage kinetics of SCAT3. This study shows that FRET technique based on GFPs could be used to study the mechanism of PDT-induced apoptosis in living cells.

Paper Details

Date Published: 4 April 2005
PDF: 8 pages
Proc. SPIE 5704, Genetically Engineered and Optical Probes for Biomedical Applications III, (4 April 2005); doi: 10.1117/12.588963
Show Author Affiliations
Yunxia Wu, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Qun Chen, South China Normal Univ. (China)
Tongsheng Chen, South China Normal Univ. (China)
Yonghong Tang, South China Normal Univ. (China)
Qingling Wan, South China Normal Univ. (China)


Published in SPIE Proceedings Vol. 5704:
Genetically Engineered and Optical Probes for Biomedical Applications III
Darryl J. Bornhop; Samuel I. Achilefu; Ramesh Raghavachari; Alexander P. Savitsky, Editor(s)

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