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Proceedings Paper

Spectral (optical) and mechanical responses of fresh and cryopreserved issued arteries
Author(s): Emilie Pery; Walter C.P.M. Blondel; Jean-Christophe Goebel; Jacques Didelon; Francois Guillemin
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Paper Abstract

Cryopreservation is the only method for conserving blood vessels as future allografts with biological immunity controls. Although it affects vessels mechanical structure, no biomechanical integrity simple test is available today. Biological tissues optical properties characterization by spectroscopic methods is of interest due to their types or natures variations. Collected data complementarity contributes to "photodiagnosis" applicative prospects (cancer, vascular...). Pig carotid artery rings were tested after excision and after one month cryopreservation. An uniaxial mechanical testing device was used for ring stretching, and elongation and axial forces measurement. Circumferential large strains and stresses were calculated. Simultaneously, each artery ring optical characteristics was measured using fibered autofluorescence and elastic scattering spectrometers. Mechanical results showed nonlinear strain/stress curves and large deformations in good agreement with other referenced works. Significant differences (p<0.05) between fresh and cryopreserved rings mechanical properties were noticed. Elastic scattering spectra intensity variations were well correlated with artery mechanical properties. The standardized autofluorescence spectra were more clearly correlated with anatomo-histological changes due to cryopreservation, providing rather accurate differentiation between fresh and cryopreserved samples. This study offers a new perspective to detect changes of cryopreserved arterial samples mechanical properties. Coupling mechanical tests (uniaxial traction of arterial rings) and optical spectroscopic measurements (autofluorescence, elastic scattering) is the driving point: it allows correlating mechanical modifications and spectral variations of artery rings before and after cryopreservation. Ultimately, this new approach could help developping a device allowing non-invasive, atraumatic and contactless optical examinations of arterial graft to assess its mechanical state before reimplantation.

Paper Details

Date Published: 15 April 2005
PDF: 9 pages
Proc. SPIE 5695, Optical Interactions with Tissue and Cells XVI, (15 April 2005); doi: 10.1117/12.588636
Show Author Affiliations
Emilie Pery, CRAN INPL (France)
Walter C.P.M. Blondel, CRAN INPL (France)
Jean-Christophe Goebel, CRAN INPL (France)
Jacques Didelon, CRAN INPL (France)
Ctr. Alexis Vautrin (France)
Francois Guillemin, CRAN INPL (France)
Ctr. Alexis Vautrin (France)

Published in SPIE Proceedings Vol. 5695:
Optical Interactions with Tissue and Cells XVI
Steven L. Jacques; William P. Roach, Editor(s)

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