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Proceedings Paper

Fluorescence detection of proteins released by Bacillus subtilis spores during heat shock germination
Author(s): Alexandra Alimova; Alvin Katz; Misu Paul; Elizabeth Rudolph; Paul Gottlieb; J. C. Steiner; Robert R. Alfano
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Paper Abstract

Fluorescence and absorption spectroscopy were performed on Bacillus subtilis spores which were heat treated at temperatures ranging from 20°C to 90°C. The tryptophan (trp) emission from the spore suspensions treated at temperatures greater than 60°C was shifted towards longer wavelength as compared to the spores which were heat treated at lower temperatures. These spectral changes were the result of proteins released by the spores into the suspension. Trp residues in the emitted proteins are in a more polar environment and therefore exhibit a larger Stokes shift. Fluorescence and absorption measurements show that the concentration of proteins in the supernatant was greater for spores treated at higher temperatures. Electrophoresis gel analysis showed the presence of a 47 KDa protein in the supernatant.

Paper Details

Date Published: 1 April 2005
PDF: 4 pages
Proc. SPIE 5692, Advanced Biomedical and Clinical Diagnostic Systems III, (1 April 2005); doi: 10.1117/12.588592
Show Author Affiliations
Alexandra Alimova, CUNY/City College (United States)
Alvin Katz, CUNY/City College (United States)
Misu Paul, CUNY/City College (United States)
Elizabeth Rudolph, CUNY/City College (United States)
Paul Gottlieb, CUNY/City College (United States)
J. C. Steiner, CUNY/City College (United States)
Robert R. Alfano, CUNY/City College (United States)

Published in SPIE Proceedings Vol. 5692:
Advanced Biomedical and Clinical Diagnostic Systems III
Tuan Vo-Dinh; Warren S. Grundfest; David A. Benaron; Gerald E. Cohn, Editor(s)

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