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Proceedings Paper

Refractive index changes during germination of bacillus subtilis spores
Author(s): Alvin Katz; Alexandra Alimova; Min Xu; Elizabeth Rudolph; Paul Gottlieb; J. C. Steiner; Robert R. Alfano
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Paper Abstract

Light transmission measurements in the wavelength range of 400 to 1000 nm were performed on Bacillus subtilis spores at periodic time intervals after heat-shock induced activation. The Gaussian ray approximation, using a concentric sphere model for the spore coat and spore core, was used to calculate the scattering cross-section of the spores. Analysis of transmission spectra determined that the refractive index of the spore core was 1.515. In the three hours following heat shock, the core refractive index decreased to 1.39, and subsequently remained constant. During the measurements, the spore radius increased from 0.38 microns to 0.6 microns. The results were confirmed by phase contrast microscopy.

Paper Details

Date Published: 1 April 2005
PDF: 4 pages
Proc. SPIE 5692, Advanced Biomedical and Clinical Diagnostic Systems III, (1 April 2005); doi: 10.1117/12.588590
Show Author Affiliations
Alvin Katz, CUNY/City College (United States)
Alexandra Alimova, CUNY/City College (United States)
Min Xu, CUNY/City College (United States)
Elizabeth Rudolph, CUNY/City College (United States)
Paul Gottlieb, CUNY/City College (United States)
J. C. Steiner, CUNY/City College (United States)
Robert R. Alfano, CUNY/City College (United States)

Published in SPIE Proceedings Vol. 5692:
Advanced Biomedical and Clinical Diagnostic Systems III
Tuan Vo-Dinh; Warren S. Grundfest; David A. Benaron; Gerald E. Cohn, Editor(s)

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