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Proceedings Paper

NIR-integrating sphere spectroscopy to determine various blood properties
Author(s): Martina Meinke; Moritz Friebel; Ingo H. Gersonde; Gerhard J. Muller
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Paper Abstract

Near infrared integrating sphere spectroscopy and chemometric multivariate calibration were applied to determine hematocrit (HCT) and oxygen saturation (O2Sat) of circulating human blood. The diffuse reflectance were measured and the partial least square method (PLS) was used for calibration considering different wavelength ranges. The HCT and the O2Sat could be predicted with a root mean square error (PRMSE) of 1.9% and 2.8% respectively, using PLS. Each parameter was adjusted to various levels, and three measurement series from blood of three different donors were carried out for the calibration with the PLS. The calibration includes changes in hemolysis and osmolarity as well as inter-individual differences in cell dimensions and hemoglobin content. Prediction of hemolysis was also possible for one blood sample with a PRMSE of 0.8%.

Paper Details

Date Published: 7 March 2005
PDF: 8 pages
Proc. SPIE 5702, Optical Diagnostics and Sensing V, (7 March 2005); doi: 10.1117/12.588495
Show Author Affiliations
Martina Meinke, Charite Universitatsmedizin Berlin (Germany)
Moritz Friebel, Laser- und Medizin-Technologie GmbH, Berlin (Germany)
Ingo H. Gersonde, Charite Univ. Hospital (Germany)
Gerhard J. Muller, Charite Universitatsmedizin Berlin (Germany)

Published in SPIE Proceedings Vol. 5702:
Optical Diagnostics and Sensing V
Alexander V. Priezzhev; Gerard L. Cote, Editor(s)

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