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Proceedings Paper

Fluorescence spectroscopy for tongue carcinoma detection: study in an animal model
Author(s): Cristina Kurachi; Carla Raquel Fontana; Claudia Adriana Sousa Melo; Luiz Eduardo Blumer Rosa; Vanderlei Salvador Bagnato
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Paper Abstract

Fluorescence diagnosis of malignant lesions has been showed as an attractive optical technique due especially to its real-time response and a more objective and quantitative evaluation. Even though the oral cavity allows a direct examination many lesions are diagnosed when it is already in advanced stage, compromising the patient prognosis. In this study, the fluorescence spectroscopy was used to the detection of chemically induced carcinoma at the lateral border of the tongue in a hamster model. Two excitations wavelengths in visible region were applied: 442 and 532 nm. All the spectra results were analyzed comparing with the histopathological diagnosis. The better results were achieved with the 442 nm laser excitation. The spectra from carcinoma showed new emission bands and these were used to determined different ratios for a quantitative analysis. Using the 625-645 nm fluorescence range under 442 nm excitation (A3 coefficient) the percentage of false negative was of 9.1%, however the false positive percentage was of 18.5%. The 532 nm excitation provided a better normal tissue detection compared to 442 nm excitation. The ideal clinical condition is probably the use of multiple wavelengths excitation for a broader tissue fluorescence investigation.

Paper Details

Date Published: 8 April 2005
PDF: 8 pages
Proc. SPIE 5689, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIV, (8 April 2005); doi: 10.1117/12.588222
Show Author Affiliations
Cristina Kurachi, Instituto de Fisica de Sao Carlos, Univ. de Sao Paulo (Brazil)
Carla Raquel Fontana, Instituto de Fisica de Sao Carlos, Univ. de Sao Paulo (Brazil)
Claudia Adriana Sousa Melo, Instituto de Fisica de Sao Carlos, Univ. de Sao Paulo (Brazil)
Luiz Eduardo Blumer Rosa, Faculdade de Odontologia de Sao Jose dos Campos/Univ. Estadual Paulista (Brazil)
Vanderlei Salvador Bagnato, Instituto de Fisica de Sao Carlos, Univ. de Sao Paulo (Brazil)


Published in SPIE Proceedings Vol. 5689:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIV
David Kessel, Editor(s)

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