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Proceedings Paper

Adaptive silver films toward bio-array applications
Author(s): Vladimir P. Drachev; Meena L. Narasimhan; Hsiao-Kuan Yuan; Mark D. Thoreson; Yong Xie; V. Joe Davisson; Vladimir M. Shalaev
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Paper Abstract

Adaptive silver films (ASFs) have been studied as a substrate for protein microarrays. Vacuum evaporated silver films fabricated at certain range of evaporation parameters allow fine rearrangement of the silver nanostructure under protein depositions in buffer solution. Proteins restructure and stabilize the ASF to increase the surface-enhanced Raman scattering (SERS) signal from a monolayer of molecules. Preliminary evidence indicates that the adaptive property of the substrates make them appropriate for protein microarray assays. Head-to-head comparisons with two commercial substrates have been performed. Protein binding was quantified on the microarray using the streptavidinCy3/biotinylated goat IgG protein pair. With fluorescence detection, the performance of ASF substrates was comparable with SuperAldehyde and SuperEpoxy substrates. Additionally, the ASF is also a SERS substrate and this provides an additional tool for analysis. It is found that the SERS spectra of the streptavidinCy5 fluorescence reporter bound to true and bound to false sites show distinct difference.

Paper Details

Date Published: 31 March 2005
PDF: 7 pages
Proc. SPIE 5703, Plasmonics in Biology and Medicine II, (31 March 2005); doi: 10.1117/12.585160
Show Author Affiliations
Vladimir P. Drachev, Purdue Univ. (United States)
Meena L. Narasimhan, Purdue Univ. (United States)
Hsiao-Kuan Yuan, Purdue Univ. (United States)
Mark D. Thoreson, Purdue Univ. (United States)
Yong Xie, Purdue Univ. (United States)
V. Joe Davisson, Purdue Univ. (United States)
Vladimir M. Shalaev, Purdue Univ. (United States)


Published in SPIE Proceedings Vol. 5703:
Plasmonics in Biology and Medicine II
Tuan Vo-Dinh; Joseph R. Lakowicz; Zygmunt K. Gryczynski, Editor(s)

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