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Proceedings Paper

Proliferative effect of green light emitting diode irradiation on chicken fibroblasts in hyperglycaemic circumstances: a preliminary in vitro study
Author(s): Elke Vinck; Barbara Cagnie; Heidi Declercq; Ria Cornelissen; Dirk Cambier
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Paper Abstract

A reduced mortality due to hyperglycaemia was noted since the development of insulin treatment for type I diabetes and various oral hypoglycaemic agents for type II diabetes. Nevertheless the chronic metabolic disorder, Diabetes Mellitus, remains an important cause of morbidity and mortality due to a series of common secondary metabolic complications. Patients with diabetes have an increased tendency to develop infections of the skin. Healing of skin lesions in diabetics evolves often relatively slow and the lesions tend to be more severe than in non-diabetics. Endeavouring to accelerate the healing process of skin lesions in diabetic patients, this preliminary in vitro study investigates the efficacy of green Light Emitting Diode (LED) irradiation on fibroblast proliferation of cells in hyperglycaemic circumstances. In an attempt to imitate the diabetic environment, embryonic chicken fibroblasts were cultured in hyperglycaemic medium (30.000mg Glucose per litre Hanks Medium). LED irradiation was performed three consecutive days with a wavelength of 540 nm and a power output of 10 mW, at 0,6 cm distance from the fibroblasts. Each treatment lasted 3 minutes, resulting in a surface energy density of 0,2 J/cm2. Statistical analysis revealed that LED irradiation at the applied parameters induced a higher rate of proliferation in hyperglycaemic circumstances after irradiation than in the same circumstances without irradiation. Regarding these results the effectiveness of green LED irradiation on cells in hyperglycaemic circumstances is proven. To ensure the effectiveness and to evaluate the value of LED irradiation in vivo, further research is required.

Paper Details

Date Published: 10 September 2004
PDF: 6 pages
Proc. SPIE 5610, Laser Florence 2003: A Window on the Laser Medicine World, (10 September 2004); doi: 10.1117/12.584395
Show Author Affiliations
Elke Vinck, Ghent Univ. (Belgium)
Barbara Cagnie, Ghent Univ. (Belgium)
Heidi Declercq, Ghent Univ. (Belgium)
Ria Cornelissen, Ghent Univ. (Belgium)
Dirk Cambier, Ghent Univ. (Belgium)

Published in SPIE Proceedings Vol. 5610:
Laser Florence 2003: A Window on the Laser Medicine World
Leonardo Longo; Alfons G. Hofstetter; Mihail-Lucian Pascu; Wilhelm R. A. Waidelich, Editor(s)

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